Isolera Flash Purification Systems

Isolera™ Spektra is a high performance ACI™ system and new generation of purification system. It is backwards compatible as a software upgrade for existing Isolera systems.

Isolera Spektra is a world leading automated flash system designed to purify synthesis reaction mixtures, natural product extracts, and other organic compound mixtures from milligram to multi-gram quantities. It features an additional host of both labour and cost saving features such as TLC-to-step gradient, full spectral analysis, lambda all collection, 3D view and improved baseline correction algorithms, allowing the same chemistries and purifications to be run but significantly reducing the amount of solvent required and time to do so. The systems are available in a number of different configurations and are so intelligent they will also recommend the optimum cartridge size for your sample.

We belive in providing value, so Isolera Spektra systems embrace ACI™, a revolutionary advancement, converting traditional regular flash from simple purification to the fastest, greenest, and most economical way to reliably isolate pure compounds for organic synthesis. ACI offerred as standard on Isolera systems.

Isolera™ Spektra One

Is a single cartridge flash purification system just right for use by one or two chemists who need to perform 1–5 purifications per day. Isolera Spektra One is compatible with all Biotage flash cartridges, including Biotage ZIP®, Biotage ZIP Sphere®, Biotage SNAP® and the gold standard in flash cartridges, Biotage SNAP Ultra®.

Isolera™ Spektra Four

Is ideal for laboratories where 2–5 chemists are sharing a single flash purification instrument or in a high-throughput synthesis laboratory performing up to 20 purifications per day.

Isolera™ Spektra LS

Is the definitve flash chromatography system for reliable scaling up from grams to to multi, even hundred gram scale purification, using the common Isolera interface. Enjoy all of the sophisitcated software features of the laboratory scale systems in a robust instrument built for scale-up labs. Isolera Spektra LS adds an integrated pump assisted loading mechanism for larger samples, and a funnel rack collection option which allows samples to be collected in larger fraction collection vessels.

ACI™ – Accelerated Chromatographic Isolation

Taking Flash Purification to Another Level Biotage has developed ACI™ Accelerated Chromatographic Isolation, a revolutionary advancement converting regular flash from simple purification to the fastest, greenest, and most economical way to reliably isolate pure compounds for organic synthesis.


Fast and Efficient
With running costs and time a concern in most labs, ACI™ now promises to take users to the end point even faster and even more efficiently. Isolera™ systems already recommend cartridges based on the sample size and your TLC data; they work out the best solvent gradient based on compound, and provide real time indication of eluting compounds, using UV, ELSD or Mass Detection. They subtract baseline signals automatically, so chemists can be confident that the correct fractions are collected.

ACI radically improves this. Traditional purification taking more than 15 minutes is reduced to five minutes; a 250 mg laboratory scale experiment can be purified in less than three minutes, even faster than the TLC used to optimize the separation.

ACI works seamlessly with the Isolera system, a simple wizard guide chemists through the system, beneficial for those of you whose expertise or priorities lie in the synthesis rather than purification.

ACI evolves flash purification to a totally new animal. Much faster, more powerful, more accurate and much greener. We believe that you will never need to buy another standard flash system again.


Achieve up to 30% solvent savings with Gradient Optimization “GO”
Step-gradients can be short and powerful providing separations for one or more compounds but can be challenging to develop and optimize. Isolera Spektra provides gradient optimization through new, patent-pending TLC-to-Step Gradient technology. Using solvent and TLC Rf values, Isolera Spektra builds a gradient to separate all the compounds (up to 6) in the sample. This new technology will also provide cartridge selection guidance based on the cartridge loading capacity and purification speed. The step gradient can also be used to isolate a targeted compound reducing run time and solvent use further.

Advanced λ-All detection
Many compounds being purified have unknown absorbance spectra. The Isolera™ Spektra λ-All function records all available wavelengths for compound detection and sums the responses to maximize sensitivity and minimize sample loss.

Digging Deeper With PDA Spectral Analysis
Isolera Spektra brings Photodiode Array (PDA) detection to flash chromatography for the first time. The full spectrum for each compound can be seen as it elutes from the cartridge – in 
real-time. This information can be used to confirm purity and compound identity. All spectra are stored and can be reviewed in 2D to verify fraction purity. Post fraction thin layer chromatography (TLC) to determine which fractions contain pure compound can be eliminated.

Baseline Correction
Many chromatographic solvents absorb UV light. Isolera Spektra uses advanced real gradient zeroing for baseline correction to eliminate background shifts. Using all wavelengths for detection with a gradient can lead to a rising baseline which will interfere with compound detection and cause inaccurate fraction collection.

3D graphics for the complete picture
Identify impurities by taking advantage of the PDA 3D chromatogram display. Chemists can view the chromatogram in terms of both elution volume and UV absorbance which provides even more purity confirmation.

Elute complex samples with the Quatro-binary gradient
Use up to four solvents in a single gradient to easily purify samples with diverse polarity. With Quatro-binary gradient capability traditional binary gradients with a limited polarity range can be adjusted to elute very lipophilic and highly polar compounds within a single purification.Or compound solubility during the separation by adding a constant amount of a co-solvent, acid, or base with the Isolera\'s advanced pump. (not available on Prime)

Maintain compound solubility with a ternary co-solvent
Isocratically pump a third solvent into any binary gradient to help maintain compound solubility eliminating potential over-pressure issues caused by precipitating compounds.

Achieve superior purification with Biotage flash cartridges
The Isolera™ Spektra One and Isolera™ Spektra Four systems can use various styles including Biotage SNAP and Biotage® SNAP Ultra with silica masses from 5 to 750 g.