Prochrom® DAC Columns by Novasep

Preparative chromatography columns - optimum performance guaranteed

1 Product description

The LC590.700.VE100 preparative High Performance Liquid Chromatography (HPLC) column is designed for cGMP operation. Any type of packing material can be used to fill the column provided the proper packing pressure is chosen to avoid destruction of the particles. It can be operated with the most common solvents used in chromatography. The column module is composed of the column itself and its compression system mounted on a stainless steel frame. It can be connected to any pumping system.
LC 590
Internal Diameter (mm) 590
Maximum Bed Length (mm) 400
Operating pressure (bar) 100
Operating Temperature (°C) 5 to 60
Compression feature Dynamic Axial Compression
Slurry inlets 1
Flow range (L/h) 900-1800

Although Prochrom® DAC column can be connected to any pumping system, performance is maximized when used in conjunction with our Prochrom® Hipersep chromatography systems.

2 Product Specifications

Column Column Tube 
The internal diameter of the column tube is 590 mm. The standard surface roughness of the column tube ID is 0.4 μm (15.7 μ inches). The external finish is approximately 1.6 μm (63 μ inches).
Piston and Flange
The piston and flange of the column contain a woven mesh frit (2μm nominal porosity) and a flow distributor to provide even distribution of the liquid (”plug flow”) over the cross section of the packed bed. The finish of the wetted surface roughness of the piston and flange is approximately 0.8μm (30 μ inches)
The flange is sealed to the column tube with a FEP encapsulated "O"-ring and held in place with a quick release chain. The piston is sealed to the column tube with a PTFE seal. The other seals are PTFE, FEP encapsulated.
The piston’s frit is cooled down with liquid nitrogen (or carbon dioxide ice) in order to shrink it. Back at room temperature, the frit expands and fits tightly to its housing.
Hydraulic Jack
The hydraulic jack is mounted above the column tube. The hydraulic jack has a stroke length of 700 mm, which allows the user to pack beds of variable lengths. The hydraulic jack exterior is made of stainless steel.
Hydraulic pump An air driven amplification pump is used to pressurize the oil. This pressurized oil is used to move the piston down or up. The oil pressure is proportional to the air supply pressure in static conditions (piston not moving).
Reset button Pneumatic push button to reset the hydraulic unit after the emergency button has been disengaged.
Emergency Stop Reset button Emergency stop to shut down the hydraulic pump in case of emergency. Pneumatic push button to reset the
hydraulic unit after the emergency button has been disengaged
Air supply Valve Pneumatic 2 way valve, used to supply the hydraulic pump with compressed air.
Motion Selector Oil distributor Pneumatic switch to control the oil distributor in order to select the piston’s motion (up, down, stop).
Pressure Regulator Air pressure regulator to set the air pressure supply to the pneumatic amplification pump. The air pressure is linear with the oil pressure.
Filter Breather filter for the oil reservoir.
Filter Oil return filter for mounted on the oil reservoir.
Oil reservoir Reservoir for the hydraulic oil with 90 L capacity.
Level indicator Oil level indicator for the oil reservoir.
Relief valve Oil pressure relief valve to protect the column in case of an accidental over-pressure.
This relief valve can be : CODAP certified for EU version; ASME Certifed for US version.
Pressure gauge Pressure gauge indicating the oil pressure. This indication is used to determine the bed compression.
Packing valves Pneumatic 3-way ball valve to isolate the column from the pumping skid for the packing and unpacking
operations and to select solvent direction toward the flange’s or piston’s side during packing operation in
order to bleed the air and/or remove the slurry solvent.
Column state Selector Pneumatic switch to change the column state from elution mode to packing mode by actuating packing
Purge state Selector Pneumatic switch to change the purge state during packing operation from piston to flange by actuating the packing valve
Pressure Transmitter Optional pressure transmitter and its isolation valve. The pressure transmitter can be connected to the HIPERSEP unit in order to manage (record & alarm) the hydraulic oil pressure.
Jack isolation valves Manual 2-way ball valve to isolate the jack for maintenance purposes.
Sight glass Sigth glass installed on the purge line to identify liquid or air presence during packing operation.

The design of the Column is made in such a way to reduce as much dead volume as possible. All wetted parts of the column module are made of SS316L stainless steel, FEP and PTFE.
Packing valves are high pressure valves with an internal surface finish not better than 2.4 μm (94 μ-inches).
Frame is made of SS304 with an external finish at Grit 220 (about 1.6 μm Ra / 62 μ-inches Ra). External frame’s welds are grinded.
The LC590.700.VE100 is rated to be operated in ATEX Zone 2 IIB T4 hazardous area