Biotage Initiator+ Alstra™

Automated Microwave Peptide Synthesizer

Biotage® Initiator+ Alstra™ is a fully automated microwave peptide synthesizer . With functionality such as Branches, Preactivation and “Edit on the fly”, and options for UV monitoring and MAOS (organic synthesis) capability, this instrument represents best-in-class systems for both small and large scale synthesis of peptides, peptoids, PNA and peptidomimetics.

Biotage® Initiator+ Alstra™ is a fully automated microwave peptide synthesizer with built in flexibility for both small and large scale synthesis. This instrument now comes with the Alstra 1.1 software upgrade that introduces new functionality such as Branches, Preactivation,  “Edit on the fly”, and options for UV monitoring and microwave assisted organic synthesis (MAOS).



Proven Efficient and Reliable

Dr. Renato Longhi at the National Research Council of Italy, Milan, gives his view on working with Initiator+ Alstra:

“We have been working with a Biotage Initiator+ Alstra since January 2013. During this period, the instrument has proved both efficient and robust, enabling us to successfully accomplish a substantial number of synthesis (more than 1,500 coupling cycles to date (october 2013)) differing in peptide sequence and size (up to 60 residues).

We have also evaluated the instrument’s performances using different solid supports and operating in different synthetic scales (50-250 µmol). Reagent and solvent delivery is very precise, whilst synthetic protocols can be easily modified according to synthetic needs. 

Whenever minor technical issues occurred, they were promptly solved by Biotage staff. Overall, the Biotage Initiator+ Alstra is in our opinion a very efficient and reliable instrument, and we are very satisfied with it!”

Simple to Program

The system is programmed via the large 10” touch screen with drag and drop feature, using either pre-defined or custom methods. The built-in wizard makes programming a synthesis sequence effortless and the calculation table generated provides the quantities of reagents required.

Flexible and Accurate

The flexible reagent setup with modular amino acid racks and reagent bottles enables both small and large scale synthesis. Multiple coupling, and capping reagents can also be used during a synthesis. The accurate dispensing of all reagents is achieved using digital syringe pumps and the dual needle design reduces the possibility of cross contamination.

Reduce Costs

Reduce your reagent and solvent consumption using the robot liquid handler with digital syringe pumps. This also minimizes consumption and waste of expensive building blocks. Save even more by benefiting from the ability to perform synthesis on a small scale from as low as 5 µmol.

Synthesize Simple and Difficult Sequences

Use microwave irradiation and standard reagents to synthesize difficult sequences that are problematic or even impossible to synthesize using conventional methods or just make simple peptides more efficiently.

Complete Control

Fully automate all cycles and/or use semi-automated operations where the Fmoc deprotection and washing steps are automated and then manually add expensive building blocks. This gives you complete control over all reaction steps. Easily pause and re-start a synthesis to remove samples for monitoring and analysis of intermediate reaction steps.

Oscillating Mixing

Oscillating mixing within the microwave cavity ensures homogeneous heat distribution and allows for larger scale capability up to 2 mmol.


This exciting new feature is a tool that allows skilled peptide chemists to assign custom methods and to enable scheduling and visualization of their operations in order to make complex peptide modifications such as side chain modification and elaboration e.g. attachment of a dye label, MAPs, intramolecular cyclization, head-to-tail cyclization, side-chain to side-chain cyclization, disulfide bridge formation.

Microwave Assisted Organic Synthesis (MAOS) Option

Use the Alstra MAOS kit (part number 356445) to enable your system to be configured for both microwave peptide and microwave organic synthesis modes of operation with dual mode of mixing: oscillating mixing for peptide synthesis and magnetic stirring for organic synthesis.

UV Monitoring Option

Use the Alstra UV monitoring kit (part number 356444) to follow the removal of the Fmoc group during the Fmoc deprotection reaction and modify the deprotection and coupling parameters. This enables the user to automatically control the deprotection time and also the number of iterations.

Now you can choose to activate the amino acid in situ or preactivate any amino acid in a cycle as required. Preactivate any position in the sequence. Set the time required for preactivation reaction to occur.

"Edit on the Fly"

This is a very powerful feature which gives the user even more control during a peptide synthesis run and allows the user to make changes to a synthesis that is already in progress (after pausing operation), pause and check a coupling and make changes based on the result without having to reprogram the synthesis, with the additional volume of reagents required adjusted in the calculation table.

Features and Advantages

  • Microwave assisted peptide synthesis
  • Fully automated and/or semi-automated operation
  • Disposable reactor vials and amino acid vessels
  • Synthesis on 5 µmol - 2 mmol scale
  • Large touchscreen with drag and drop feature
  • User friendly and intuitive software
  • Flexible reagent setup with modular racks
  • Use pre-defined or custom methods
  • Oscillating mixing for efficient coupling
  • Precise digital syringe pumps
  • Easily pause and re-start synthesis
  • Less valves for less downtime
  • Inert gas option for microwave cavity and reagents
  • Compact footprint